Development of Loop-Mediated Isothermal Amplification for Detection of Leifsonia xyli subsp. xyli in SugarcaneReportar como inadecuado

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BioMed Research InternationalVolume 2013 2013, Article ID 357692, 8 pages

Research Article

Key Lab of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China

USDA-ARS, Sugarcane Research Unit, Houma, LA 70360, USA

Received 15 February 2013; Accepted 4 April 2013

Academic Editor: Jozef Anné

Copyright © 2013 Jing Liu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Ratoon stunt, caused by the xylem-limited coryneform bacterium Leifsonia xyli subsp. xyli Lxx, is a deep bacteriosis and prevalent in most of sugarcane-producing countries. Based on loop-mediated isothermal amplification LAMP, we developed a method for detecting Lxx. The major advantages of the LAMP method are visual judgment by color and time saving with only 60 min for identification of Lxx and without the need for costly PCR apparatus and gel scanner. In the present study, positive and negative samples detected by the LAMP method were clearly distinguishable. When total DNA extracted from internode juice was used as the template, the sensitivity of LAMP was 10 times higher than that of the conventional PCR detection. The LAMP assay is a highly specific, rapid, and sensitive method for the diagnosis of ratoon stunt caused by Lxx in sugarcane. This is the first report of LAMP-based assay for the detection of Lxx in sugarcane.

Autor: Jing Liu, Liping Xu, Jinlong Guo, Rukai Chen, Michael Paul Grisham, and Youxiong Que



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