Ikaros and RAG-2-Mediated Antisense Transcription Are Responsible for Lymphocyte-Specific Inactivation of NWC PromoterReportar como inadecuado




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Recombination activating gene-2 RAG-2 and NWC are strongly evolutionarily conserved overlapping genes which are convergently transcribed. In non-lymphoid cells the NWC promoter is active whereas in lymphocytes it is inactive due to the DNA methylation. Analysing the mechanism responsible for lymphocyte-specific methylation and inactivation of NWC promoter we found that Ikaros, a lymphocyte-specific transcription factor, acts as a repressor of NWC promoter - thus identifying a new Ikaros target - but is insufficient for inducing its methylation which depends on the antisense transcription driven by RAG-2 promoter. Possible implications of these observations for understanding evolutionary mechanisms leading to lymphocyte specific expression of RAG genes are discussed.



Autor: Agnieszka Łaszkiewicz , Łukasz Bzdzion , Monika Kasztura, Łukasz Śnieżewski, Sylwia Janik, Paweł Kisielow, Małgorzata Cebr

Fuente: http://plos.srce.hr/



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