NMR Spectroscopy of Human Eye Tissues: A New Insight into Ocular BiochemistryReportar como inadecuado

NMR Spectroscopy of Human Eye Tissues: A New Insight into Ocular Biochemistry - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

The Scientific World Journal - Volume 2014 2014, Article ID 546192, 9 pages -

Research Article

Department of Neuroscience, Faculty of Medicine, Norwegian University of Science and Technology, Edvard Griegs Gate. 8, 3. Etg., 7489 Trondheim, Norway

Department of Experimental Pharmacology, Mossakowski Medical Research Centre, Polish Academy of Sciences, 02-106 Warsaw, Poland

Department of Ophthalmology, Medical University of Silesia, District Railway Hospital, 40-001 Katowice, Poland

Department of Ophthalmology, University Hospital, 7006 Trondheim, Norway

Received 31 July 2014; Revised 27 September 2014; Accepted 19 October 2014; Published 26 November 2014

Academic Editor: Michael P. Fautsch

Copyright © 2014 Tomasz Kryczka et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Background. The human eye is a complex organ whose anatomy and functions has been described very well to date. Unfortunately, the knowledge of the biochemistry and metabolic properties of eye tissues varies. Our objective was to reveal the biochemical differences between main tissue components of human eyes. Methods. Corneas, irises, ciliary bodies, lenses, and retinas were obtained from cadaver globes 0-1-2 hours postmortem of 6 male donors age: 44–61 years. The metabolic profile of tissues was investigated with HR MAS

H NMR spectroscopy. Results. A total of 29 metabolites were assigned in the NMR spectra of the eye tissues. Significant differences between tissues were revealed in contents of the most distant eye-tissues, while irises and ciliary bodies showed minimal biochemical differences. ATP, acetate, choline, glutamate, lactate, myoinositol, and taurine were identified as the primary biochemical compounds responsible for differentiation of the eye tissues. Conclusions. In this study we showed for the first time the results of the analysis of the main human eye tissues with NMR spectroscopy. The biochemical contents of the selected tissues seemed to correspond to their primary anatomical and functional attributes, the way of the delivery of the nutrients, and the location of the tissues in the eye.

Autor: Tomasz Kryczka, Edward Wylęgała, Dariusz Dobrowolski, and Anna Midelfart

Fuente: https://www.hindawi.com/


Documentos relacionados