Temporal Bacteriostatic Effect and Growth Factor Loss in Equine Platelet Components and Plasma Cultured with Methicillin-Sensitive and Methicillin-Resistant Staphylococcus aureus: A Comparative In Vitro StudyReportar como inadecuado




Temporal Bacteriostatic Effect and Growth Factor Loss in Equine Platelet Components and Plasma Cultured with Methicillin-Sensitive and Methicillin-Resistant Staphylococcus aureus: A Comparative In Vitro Study - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Veterinary Medicine International - Volume 2014 2014, Article ID 525826, 8 pages -

Research ArticleGrupo de Investigación Terapia Regenerativa, Departamento de Salud Animal, Universidad de Caldas, Calle 65 No. 26-10, Manizales, Colombia

Received 28 August 2014; Accepted 3 November 2014; Published 24 November 2014

Academic Editor: Francesca Mancianti

Copyright © 2014 Catalina López et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The aims were 1 to evaluate the bacteriostatic effect of platelet-rich plasma PRP, platelet-rich gel PRG, leukocyte-poor plasma LPP, leukocyte-poor gel LPG, plasma, and heat-inactivated plasma IP on both methicillin-sensitive Staphylococcus aureus MSSA and methicillin-resistant Staphylococcus aureus MRSA over a period of 24 h; 2 to determine and to compare the concentrations and degradation over time of platelet factor 4 PF-4, transforming growth factor beta 1 TGF-β1, and platelet-derived growth factor isoform BB PDGF-BB; and 3 to identify any correlations between MSSA and MRSA growth and either the cellular, PF-4, TGF-β1, or PDGF-BB concentrations in the blood components. PRP and its byproducts from 18 horses were obtained by the tube method. All blood components were cultured with either MSSA or MRSA. Bacterial growth, PF-4, TGF-β1, and PDGF-BB were determined at 6 h and 24 h. At six hours, bacterial growth was significantly inhibited by all blood components, with the exception of IP. MSSA was more sensitive to the treatments than MRSA. At 24 hours, bacterial growth was significantly higher in IP. MRSA bacterial growth was significantly higher in PRP, LPP, and plasma when compared to MSSA. Growth factor concentrations were not significantly affected by bacteria.





Autor: Catalina López, María E. Álvarez, and Jorge U. Carmona

Fuente: https://www.hindawi.com/



DESCARGAR PDF




Documentos relacionados