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Stem Cells International - Volume 2016 2016, Article ID 2514917, 13 pages -

Research Article

Mossakowski Medical Research Centre, Polish Academy of Sciences, 5 Pawinskiego Street, 02-106 Warsaw, Poland

Department of Plastic Surgery at the Medical Center for Postgraduate Education, Warsaw School of Medicine, 01-828 Warsaw, Poland

Received 22 April 2016; Revised 30 June 2016; Accepted 11 July 2016

Academic Editor: Phuc Van Pham

Copyright © 2016 Wioletta Lech et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Mesenchymal stem cells MSC exhibit enormous heterogeneity which can modify their regenerative properties and therefore influence therapeutic effectiveness as well as safety of these cells transplantation. In addition the high phenotypic plasticity of MSC population makes it enormously sensitive to any changes in environmental properties including fluctuation in oxygen concentration. We have shown here that lowering oxygen level far below air atmosphere has a beneficial impact on various parameters characteristic for umbilical cord Wharton Jelly- WJ- MSC and adipose tissue- AD- derived MSC cultures. This includes their cellular composition, rate of proliferation, and maintenance of stemness properties together with commitment to cell differentiation toward mesodermal and neural lineages. In addition, the culture genomic stability increased significantly during long-term cell passaging and eventually protected cells against spontaneous transformation. Also by comparing of two routinely used methods of MSCs isolation mechanical versus enzymatic we have found substantial divergence arising between cell culture properties increasing along the time of cultivation in vitro. Thus, in this paper we highlight the urgent necessity to develop the more sensitive and selective methods for prediction and control cells fate and functioning during the time of growth in vitro.

Autor: Wioletta Lech, Anna Figiel-Dabrowska, Anna Sarnowska, Katarzyna Drela, Patrycja Obtulowicz, Bartlomiej Henryk Noszczyk, Leon



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