Transcriptional Response of Musca domestica Larvae to Bacterial InfectionReportar como inadecuado

Transcriptional Response of Musca domestica Larvae to Bacterial Infection - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

The house fly Musca domestica, a cosmopolitan dipteran insect, is a significant vector for human and animal bacterial pathogens, but little is known about its immune response to these pathogens. To address this issue, we inoculated the larvae with a mixture of Escherichia coli and Staphylococcus aureus and profiled the transcriptome 6, 24, and 48 h thereafter. Many genes known to controlling innate immunity in insects were induced following infection, including genes encoding pattern recognition proteins PGRPs, various components of the Toll and IMD signaling pathways and of the proPO-activating and redox systems, and multiple antimicrobial peptides. Interestingly, we also uncovered a large set of novel immune response genes including two broad-spectrum antimicrobial peptides muscin and domesticin, which might have evolved to adapt to house-fly-s unique ecological environments. Finally, genes mediating oxidative phosphorylation were repressed at 48 h post-infection, suggesting disruption of energy homeostasis and mitochondrial function at the late stages of infection. Collectively, our data reveal dynamic changes in gene expression following bacterial infection in the house fly, paving the way for future in-depth analysis of M. domestica-s immune system.

Autor: Ting Tang, Xiang Li, Xue Yang, Xue Yu, Jianhui Wang, Fengsong Liu , Dawei Huang



Documentos relacionados