Fabrication of an Amperometric Flow-Injection Microfluidic Biosensor Based on Laccase for In Situ Determination of Phenolic CompoundsReportar como inadecuado




Fabrication of an Amperometric Flow-Injection Microfluidic Biosensor Based on Laccase for In Situ Determination of Phenolic Compounds - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

BioMed Research International - Volume 2015 2015, Article ID 845261, 9 pages -

Research ArticleCMUA, Department of Electrical and Electronics Engineering, University of Los Andes, Cra 1 E No. 19 A-40, Bogota, Colombia

Received 18 February 2015; Revised 30 April 2015; Accepted 1 May 2015

Academic Editor: Jinsong Ren

Copyright © 2015 Juan C. Gonzalez-Rivera and Johann F. Osma. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

We aim to develop an in situ microfluidic biosensor based on laccase from Trametes pubescens with flow-injection and amperometry as the transducer method. The enzyme was directly immobilized by potential step chronoamperometry, and the immobilization was studied using cyclic voltammetry and electrochemical impedance spectroscopy. The electrode response by amperometry was probed using ABTS and syringaldazine. A shift of interfacial electron transfer resistance and the electron transfer rate constant from 18.1 kΩ to 3.9 MΩ and 4.6 × 10

 cm s

to 2.1 × 10

 cm s

, respectively, evidenced that laccase was immobilized on the electrode by the proposed method. We established the optimum operating conditions of temperature 55°C, pH 4.5, injection flow rate 200 µL min

, and applied potential 0.4 V. Finally, the microfluidic biosensor showed better lower limit of detection 0.149 µM and sensitivity 0.2341 nA µM

for ABTS than previous laccase-based biosensors and the in situ operation capacity.





Autor: Juan C. Gonzalez-Rivera and Johann F. Osma

Fuente: https://www.hindawi.com/



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