Whole Genome Sequencing and Analysis of Plant Growth Promoting Bacteria Isolated from the Rhizosphere of Plantation Crops Coconut, Cocoa and ArecanutReportar como inadecuado




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Coconut, cocoa and arecanut are commercial plantation crops that play a vital role in the Indian economy while sustaining the livelihood of more than 10 million Indians. According to 2012 Food and Agricultural organization-s report, India is the third largest producer of coconut and it dominates the production of arecanut worldwide. In this study, three Plant Growth Promoting Rhizobacteria PGPR from coconut CPCRI-1, cocoa CPCRI-2 and arecanut CPCRI-3 characterized for the PGP activities have been sequenced. The draft genome sizes were 4.7 Mb 56% GC, 5.9 Mb 63.6% GC and 5.1 Mb 54.8% GB for CPCRI-1, CPCRI-2, CPCRI-3, respectively. These genomes encoded 4056 CPCRI-1, 4637 CPCRI-2 and 4286 CPCRI-3 protein-coding genes. Phylogenetic analysis revealed that both CPCRI-1 and CPCRI-3 belonged to Enterobacteriaceae family, while, CPCRI-2 was a Pseudomonadaceae family member. Functional annotation of the genes predicted that all three bacteria encoded genes needed for mineral phosphate solubilization, siderophores, acetoin, butanediol, 1-aminocyclopropane-1-carboxylate ACC deaminase, chitinase, phenazine, 4-hydroxybenzoate, trehalose and quorum sensing molecules supportive of the plant growth promoting traits observed in the course of their isolation and characterization. Additionally, in all the three CPCRI PGPRs, we identified genes involved in synthesis of hydrogen sulfide H2S, which recently has been proposed to aid plant growth. The PGPRs also carried genes for central carbohydrate metabolism indicating that the bacteria can efficiently utilize the root exudates and other organic materials as energy source. Genes for production of peroxidases, catalases and superoxide dismutases that confer resistance to oxidative stresses in plants were identified. Besides these, genes for heat shock tolerance, cold shock tolerance and glycine-betaine production that enable bacteria to survive abiotic stress were also identified.



Autor: Alka Gupta , Murali Gopal , George V. Thomas, Vinu Manikandan, John Gajewski, George Thomas, Somasekar Seshagiri, Stephan C. Schu

Fuente: http://plos.srce.hr/



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