Qishenyiqi Protects Ligation-Induced Left Ventricular Remodeling by Attenuating Inflammation and Fibrosis via STAT3 and NF-κB Signaling PathwayReportar como inadecuado




Qishenyiqi Protects Ligation-Induced Left Ventricular Remodeling by Attenuating Inflammation and Fibrosis via STAT3 and NF-κB Signaling Pathway - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Aim

Qi-shen-yi-qi QSYQ, a formula used for the routine treatment of heart failure HF in China, has been demonstrated to improve cardiac function through down-regulating the activation of the Renin-Angiotensin-Aldosterone System RAAS. However, the mechanisms governing its therapeutic effects are largely unknown. The present study aims to demonstrate that QSYQ treatment can prevent left ventricular remodeling in heart failure by attenuating oxidative stress and inhabiting inflammation.

Methods

Sprague-Dawley SD rats were randomly divided into 6 groups: sham group, model group LAD coronary artery ligation, QSYQ group with high dosage, middle dosage and low dosage LAD ligation and treated with QSYQ, and captopril group LAD ligation and treated with captopril as the positive drug. Indicators of fibrosis Masson, MMPs, and collagens and inflammation factors were detected 28 days after surgery.

Results

Results of hemodynamic alterations dp-dt value in the model group as well as other ventricular remodeling VR markers, such as MMP-2, MMP-9, collagen I and III elevated compared with sham group. VR was accompanied by activation of RAAS angiotensin II and NADPHoxidase. Levels of pro-inflammatory cytokines TNF-α, IL-6 in myocardial tissue were also up-regulated. Treatment of QSYQ improved cardiac remodeling through counter-acting the aforementioned events. The improvement of QSYQ was accompanied with a restoration of angiotensin II-NADPHoxidase-ROS-MMPs pathways. In addition -therapeutic- QSYQ administration can reduce both TNF-α-NF-B and IL-6-STAT3 pathways, respectively, which further proves the beneficial effects of QSYQ.

Conclusions

Our study demonstrated that QSYQ protected LAD ligation-induced left VR via attenuating AngII -NADPH oxidase pathway and inhabiting inflammation. These findings provide evidence as to the cardiac protective efficacy of QSYQ to HF and explain the beneficial effects of QSYQ in the clinical application for HF.



Autor: Chun Li , Yong Wang , Qi Qiu , Tianjiao Shi, Yan Wu, Jing Han, Xingyun Chai, Wei Wang

Fuente: http://plos.srce.hr/



DESCARGAR PDF




Documentos relacionados