Acquisition of Cry1Ac Protein by Non-Target Arthropods in Bt Soybean FieldsReportar como inadecuado




Acquisition of Cry1Ac Protein by Non-Target Arthropods in Bt Soybean Fields - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Soybean tissue and arthropods were collected in Bt soybean fields in China at different times during the growing season to investigate the exposure of arthropods to the plant-produced Cry1Ac toxin and the transmission of the toxin within the food web. Samples from 52 arthropod species-taxa belonging to 42 families in 10 orders were analysed for their Cry1Ac content using enzyme-linked immunosorbent assay ELISA. Among the 22 species-taxa for which three samples were analysed, toxin concentration was highest in the grasshopper Atractomorpha sinensis and represented about 50% of the concentration in soybean leaves. Other species-taxa did not contain detectable toxin or contained a concentration that was between 1 and 10% of that detected in leaves. These Cry1Ac-positive arthropods included a number of mesophyll-feeding Hemiptera, a cicadellid, a curculionid beetle and, among the predators, a thomisid spider and an unidentified predatory bug belonging to the Anthocoridae. Within an arthropod species-taxon, the Cry1Ac content sometimes varied between life stages nymphs-larvae vs. adults and sampling dates before, during, and after flowering. Our study is the first to provide information on Cry1Ac-expression levels in soybean plants and Cry1Ac concentrations in non-target arthropods in Chinese soybean fields. The data will be useful for assessing the risk of non-target arthropod exposure to Cry1Ac in soybean.



Autor: Huilin Yu, Jörg Romeis, Yunhe Li, Xiangju Li, Kongming Wu

Fuente: http://plos.srce.hr/



DESCARGAR PDF




Documentos relacionados