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Croatian medical journal, Vol.55 No.4 August 2014. -

The generally accepted hypothesis on cerebrospinal fluid

CSF hydrodynamics suggests that CSF is actively formed

mainly by the choroid plexuses, circulates unidirectionally

along the brain ventricles and subarachnoid space, and is

passively absorbed mainly into the dural venous sinuses.

CSF formation rate Vf has been extensively studied using

the ventriculo-cisternal perfusion technique and the results

have been used as the key evidence confirming the

mentioned hypothesis. This technique and the equation

for Vf calculation are based on the assumption that the dilution

of the indicator substance is a consequence of the

newly formed CSF, ie, that a higher CSF formation rate will

result in a higher degree of dilution. However, it has been

experimentally shown that the indicator substance dilution

inside the CSF system does not occur because of a

-newly formed- CSF, but as consequence of a number of

other factors departure of substances into the surrounding

tissue, flowing around the collecting cannula into the

cortical and spinal subarachnoid space, departure into the

contralateral ventricle, etc. This technique allows -calculation-

of the CSF formation even in dead animals, in an in vitro

model, and in any other part of the CSF system outside

the ventricles that is being perfused. Therefore, this method

is indirect and any dilution of the indicator substance

in the perfusate caused by other reasons would result in

questionable and often contradictory conclusions regarding

CSF formation rates.



Autor: Darko Orešković - ; Ruđer Bošković Institute Department of Molecular Biology, Zagreb, Croatia Marijan Klarica - ; Department

Fuente: http://hrcak.srce.hr/



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