Effect of Pregnancy on Anti-HEV Antibody Titres, Plasma Cytokines and the Corresponding Gene Expression Levels in the PBMCs of Patients Presenting with Self-Recovering Clinical and Subclinical Hepatitis EReportar como inadecuado




Effect of Pregnancy on Anti-HEV Antibody Titres, Plasma Cytokines and the Corresponding Gene Expression Levels in the PBMCs of Patients Presenting with Self-Recovering Clinical and Subclinical Hepatitis E - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

High mortality in pregnant women PR is a characteristic of hepatitis E in developing countries. To understand the pathogenesis of HEV infection in self-limiting disease during pregnancy, we compared clinical PR-patients and subclinical-HEV-infections in pregnant women in the first SC-PR-1 and later 2nd and 3rd, SC-PR-2+3 trimesters with the respective healthy controls and acute non-PR patients. The SC-PR-2+3 exhibited lower ALT, bilirubin levels, anti-HEV-IgM-IgG titres than the acute-PR-non-PR-patients p<0.05–0.0001. IFNγ-IL4ratios indicated Th2-Th1 bias in non-PR and PR-patients respectively. Raised levels of 10-20 plasma cytokines in the non-PR-patients reflect predominant inflammatory response, unaltered- IFNγ-reduced-IFNα responses and a robust chemokine secretion. On contrary, the acute-PR-patients exhibited drastic reduction in majority of the cytokines relative to in the non-PR-patients. Importantly, diminished or unaltered response was noted in the acute-PR-group when compared to the corresponding controls. The only exception was sIL2RA, increasing in both patient categories. Of the 14 genes evaluated, the expression of IFNγ-IL10-IL1A-IL7-CCL2-CCL3-CXCL8-CXCL10 was higher in the non-PR patients. Of these, the expression of IFNγ-IL10-IL1A-CCL2-CCL3-CXCL8 and, additionally, IL2-IL6-TNF genes was higher in the clinical-PRs. Almost identical pattern was noted in the control-PR-2+3 category indicating no influence of HEV infection. Comparison of patient-categories identified significant elevation of IFNγP<0.001, CCL2p<0.01, CXCL8P<0.05, IL1Bp<0.05 and IL10P<0.0001 and decrease in CXCL10<0.05 in the PR-patients. The results suggest antibody-dependent disease severity and impaired immune response in the PR patients. Higher expression of cytokine-genes in the PBMCs did not correlate with the plasma-cytokine levels in the PR-patients.



Autor: Ashwini Y. Ramdasi, Ravi P. Arya, Vidya A. Arankalle

Fuente: http://plos.srce.hr/



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