Role of Polycomb Group Proteins in the DNA Damage Response – A ReassessmentReportar como inadecuado

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A growing body of evidence suggests that Polycomb group PcG proteins, key regulators of lineage specific gene expression, also participate in the repair of DNA double-strand breaks DSBs but evidence for direct recruitment of PcG proteins at specific breaks remains limited. Here we explore the association of Polycomb repressive complex 1 PRC1 components with DSBs generated by inducible expression of the AsiSI restriction enzyme in normal human fibroblasts. Based on immunofluorescent staining, the co-localization of PRC1 proteins with components of the DNA damage response DDR in these primary cells is unconvincing. Moreover, using chromatin immunoprecipitation and deep sequencing ChIP-seq, which detects PRC1 proteins at common sites throughout the genome, we did not find evidence for recruitment of PRC1 components to AsiSI-induced DSBs. In contrast, the S2056 phosphorylated form of DNA-PKcs and other DDR proteins were detected at a subset of AsiSI sites that are predominantly at the 5′ ends of transcriptionally active genes. Our data question the idea that PcG protein recruitment provides a link between DSB repairs and transcriptional repression.

Autor: Hollie Chandler, Harshil Patel, Richard Palermo, Sharon Brookes, Nik Matthews, Gordon Peters



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