Establishment of a New Murine Elastase-Induced Aneurysm Model Combined with TransplantationReportar como inadecuado




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Introduction

The aim of our study was to develop a reproducible murine model of elastase-induced aneurysm formation combined with aortic transplantation.

Methods

Adult male mice n = 6–9 per group underwent infrarenal, orthotopic transplantation of the aorta treated with elastase or left untreated. Subsequently, both groups of mice were monitored by ultrasound until 7 weeks after grafting.

Results

Mice receiving an elastase-pretreated aorta developed aneurysms and exhibited a significantly increased diastolic vessel diameter compared to control grafted mice at 7 week after surgery 1.11±0.10 mm vs. 0.75±0.03 mm; p≤0,001. Histopathological examination revealed disruption of medial elastin, an increase in collagen content and smooth muscle cells, and neointima formation in aneurysm grafts.

Conclusions

We developed a reproducible murine model of elastase-induced aneurysm combined with aortic transplantation. This model may be suitable to investigate aneurysm-specific inflammatory processes and for use in gene-targeted animals.



Autor: Zuzanna Rowinska , Simone Gorressen , Marc W. Merx, Thomas A. Koeppel, Elisa A. Liehn , Alma Zernecke

Fuente: http://plos.srce.hr/



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