The Polymorphism in the Promoter of HSP70 Gene Is Associated with Heat Tolerance of Two Congener Endemic Bay Scallops Argopecten irradians irradians and A. i. concentricusReportar como inadecuado




The Polymorphism in the Promoter of HSP70 Gene Is Associated with Heat Tolerance of Two Congener Endemic Bay Scallops Argopecten irradians irradians and A. i. concentricus - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Background

The heat shock protein 70 HSP70 is one kind of molecular chaperones, which plays a key role in protein metabolism under normal and stress conditions.

Methodology

In the present study, the mRNA expressions of HSP70 under normal physiological condition and after acute heat stress were investigated in gills of two bay scallop populations Argopecten irradians irradians and A. i. concentricus. The heat resistant scallops A. i. concentricus showed significantly lower basal level and higher induction of HSP70 compared with that of the heat sensitive scallops A. i. irradians. The promoter sequence of HSP70 gene from bay scallop AiHSP70 was cloned and the polymorphisms within this region were investigated to analyze their association with heat tolerance. Totally 11 single nucleotide polymorphisms SNPs were identified, and four of them −967, −480, −408 and −83 were associated with heat tolerance after HWE analysis and association analysis. Based on the result of linkage disequilibrium analysis, the in vitro transcriptional activities of AiHSP70 promoters with different genotype were further determined, and the results showed that promoter from A. i. concentricus exhibited higher transcriptional activity than that from A. i. irradians P<0.05.

Conclusions

The results provided insights into the molecular mechanisms underlying the thermal adaptation of different congener endemic bay scallops, which suggested that the increased heat tolerance of A. i. concentricus compared with A. i. irradians was associated with the higher expression of AiHSP70. Meanwhile, the −967 GG, −480 AA, −408 TT and −83 AG genotypes could be potential markers for scallop selection breeding with higher heat tolerance.



Autor: Chuanyan Yang, Lingling Wang , Jingjing Wang, Qiufen Jiang, Limei Qiu, Huan Zhang, Linsheng Song

Fuente: http://plos.srce.hr/



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