Abbott RealTime HBV Assay Is More Sensitive in Detection of Low Viral Load and Little Impacted by Drug Resistant Mutation in Chronic Hepatitis B Patients under Nucleotside Analogues TherapyReportar como inadecuado




Abbott RealTime HBV Assay Is More Sensitive in Detection of Low Viral Load and Little Impacted by Drug Resistant Mutation in Chronic Hepatitis B Patients under Nucleotside Analogues Therapy - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Background

Selection of drug-resistant strains may lead to failure of HBV antiviral therapy. There is little information whether there is detection difference in drug resistant mutations between different viral load assays of HBV.

Objectives

This study is aimed to investigate whether there is drug-resistant strains related detection difference between Abbott RealTime HBV RealTime and CobasAmpliPrep-CobasTaqMan HBV assays 2.0 TaqMan.

Study Design

One hundred and thirty-four CHB patients who received HBV anti-viral therapy were enrolled. HBV virological markers were tested 3 months apart regularly. Serum HBV DNA levels were determined using the TaqMan and RealTime. YMDD rt180M and rt204V mutation was checked in patients who experienced virologic breakthrough VBT.

Results

The correlation of HBV DNA observed between the RealTime and TaqMan was good for all 571 samples R2 = 0.797; P<0.001. However, the correlation in the 434 samples with HBV DNA level <3 log10 IU-ml was not as good as in all samples R2 = 0.457. Overall, 21.5% of samples had a detection difference of ≥1 log10 IU-ml with 91.9% of these having HBV DNA level <3 log10 IU-ml. Twenty-four patients experiencedVBT. Three of these patients had acquired the YMDD mutation and exhibited discordant viral load results between the two methods tested. In each case, persistent HBV DNA was detected by RealTime and undetectable with TaqMan. Of the patients who experienced a VBT and had acquired YMDD mutation, 4.7% had undetectable HBV DNA by TaqMan while all were detectable with RealTime.

Conclusions

RealTime assay is more sensitive and is little impacted by the development of drug resistant mutation.



Autor: Ming-Lun Yeh, Chung-Feng Huang, Ching-I Huang, Shu-Fen Liu, Hua-Ling Yang, Ming-Yen Hsieh, Jee-Fu Huang, Chia-Yen Dai, Wan-Long C

Fuente: http://plos.srce.hr/



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