Effects of Cationic Antimicrobial Peptides on Liquid-Preserved Boar SpermatozoaReportar como inadecuado




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Antibiotics are mandatory additives in semen extenders to control bacterial contamination. The worldwide increase in resistance to conventional antibiotics requires the search for alternatives not only for animal artificial insemination industries, but also for veterinary and human medicine. Cationic antimicrobial peptides are of interest as a novel class of antimicrobial additives for boar semen preservation. The present study investigated effects of two synthetic cyclic hexapeptides c-WFW, c-WWW and a synthetic helical magainin II amide derivative MK5E on boar sperm during semen storage at 16°C for 4 days. The standard extender, Beltsville Thawing Solution BTS containing 250 µg-mL gentamicin standard, was compared to combinations of BTS with each of the peptides in a split-sample procedure. Examination revealed peptide- and concentration-dependent effects on sperm integrity and motility. Negative effects were more pronounced for MK5E than in hexapeptide-supplemented samples. The cyclic hexapeptides were partly able to stimulate a linear progressive sperm movement. When using low concentrations of cyclic hexapeptides 4 µM c-WFW, 2 µM c-WWW sperm quality was comparable to the standard extender over the course of preservation. C-WFW-supplemented boar semen resulted in normal fertility rates after AI. In order to investigate the interaction of peptides with the membrane, electron spin resonance spectroscopic measurements were performed using spin-labeled lipids. C-WWW and c-WFW reversibly immobilized an analog of phosphatidylcholine PC, whereas MK5E caused an irreversible increase of PC mobility. These results suggest testing the antimicrobial efficiency of non-toxic concentrations of selected cyclic hexapeptides as potential candidates to supplement-replace common antibiotics in semen preservation.



Autor: Martin Schulze , Christof Junkes, Peter Mueller, Stephanie Speck, Karin Ruediger, Margitta Dathe, Karin Mueller

Fuente: http://plos.srce.hr/



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