Enhanced activation of matrix metalloproteinase-9 correlates with the degree of papillary thyroid carcinoma infiltrationReportar como inadecuado

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Croatian medical journal, Vol.55 No.2 April 2014. -

Aim To determine whether matrix metalloproteinase-9

MMP-9 may be a useful adjunctive tool for predicting

unfavorable biological behavior of papillary thyroid carcinoma

PTC by evaluating the expression profile and proteolytic

activity of MMP-9 in PTC by different techniques

and correlating the findings with clinicopathological prognostic


Methods Immunohistochemical localization of MMP-9

was analyzed with antibodies specific for either total or active

MMP-9. Activation ratios of MMP-9 were calculated by

quantifying gel zymography bands. Enzymatic activity of

MMP-9 was localized by in situ zymography after inhibiting

MMP-2 activity.

Results Immunostaining of total and active MMP-9 was

observed in tumor tissue and occasionally in non-neoplastic

epithelium. Only active MMP-9 was significantly associated

with extrathyroid invasion, lymph-node metastasis,

and the degree of tumor infiltration P < 0.001, P = 0.004,

and P < 0.001, respectively. Gelatin zymography revealed a

correlation between the MMP-9 activation ratio and nodal

involvement, extrathyroid invasion, and the degree of tumor

infiltration. In situ zymography showed that gelatinases

exerted their activity in tumor parenchymal and stromal

cells. Moreover, after application of MMP-2 inhibitor, the remaining

gelatinase activity, corresponding to MMP-9, was

highest in cancers with the most advanced degree of tumor


Conclusions This is the first report suggesting that the

evaluation of active MMP-9 by immunohistochemistry and

determination of its activation ratio by gelatin zymography

may be a useful adjunct to the known clinicopathological

factors in predicting tumor behavior. Most important, in

situ zimography with an MMP-2 inhibitor for the first time

demonstrated a strong impact of MMP-9 activity on the

degree of tumor infiltration during PTC progression.

Autor: Ilona Marečko - ; Institute for the Application of Nuclear Energy – INEP, University of Belgrade, Zemun-Belgrade, Serbia Dubra

Fuente: http://hrcak.srce.hr/


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