Mapping B-Cell Epitopes for the Peroxidoxin of Leishmania Viannia braziliensis and Its Potential for the Clinical Diagnosis of Tegumentary and Visceral LeishmaniasisReportar como inadecuado




Mapping B-Cell Epitopes for the Peroxidoxin of Leishmania Viannia braziliensis and Its Potential for the Clinical Diagnosis of Tegumentary and Visceral Leishmaniasis - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

The search toward the establishment of novel serological tests for the diagnosis of leishmaniasis and proper differential diagnosis may represent one alternative to the invasive parasitological methods currently used to identify infected individuals. In the present work, we investigated the potential use of recombinant peroxidoxin rPeroxidoxin of Leishmania Viannia braziliensis as a potential antigen for the immunodiagnosis of human tegumentary TL and visceral leishmaniasis VL and canine visceral leishmaniasis CVL. Linear B-cell epitope mapping was performed to identify polymorphic epitopes when comparing orthologous sequences present in Trypanosoma cruzi, the agent for Chagas disease CD, and the Homo sapiens and Canis familiaris hosts. The serological assay ELISA demonstrated that TL, VL and CVL individuals showed high levels of antibodies against rPeroxidoxin, allowing identification of infected ones with considerable sensitivity and great ability to discriminate specificity between non-infected and CD individuals 98.46% and 100%; 98.18% and 95.71%; 95.79% and 100%, respectively. An rPeroxidoxin ELISA also showed a greater ability to discriminate between vaccinated and infected animals, which is an important requirement for the public campaign control of CVL. A depletion ELISA assay using soluble peptides of this B-cell epitope confirmed the recognition of these sites only by Leishmania-infected individuals. Moreover, this work identifies two antigenic polymorphic linear B-cell epitopes of L. braziliensis. Specific recognition of TL and VL patients was confirmed by significantly decreased IgG reactivity against rPeroxidoxin after depletion of peptide-1- and peptide-2-specific antibodies peptide 1: reduced by 32%, 42% and 5% for CL, ML and VL, respectively; peptide-2: reduced by 24%, 22% and 13% for CL, ML and VL, respectively and only peptide-2 for CVL reduced 9%. Overall, rPeroxidoxin may be a potential antigen for the immunodiagnosis of TL, VL or CVL, as it has a higher agreement with parasitological assays and is better than other reference tests that use soluble Leishmania antigens for diagnosing CVL in Brazil EIE-LVC, Bio-manguinhos, FIOCRUZ.



Autor: Daniel Menezes-Souza, Tiago Antônio de Oliveira Mendes, Ronaldo Alves Pinto Nagem, Thaís Teodoro de Oliveira Santos, Ana Luíza

Fuente: http://plos.srce.hr/



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