Lipopolysaccharide Induces Immune Activation and SIV Replication in Rhesus Macaques of Chinese OriginReportar como inadecuado




Lipopolysaccharide Induces Immune Activation and SIV Replication in Rhesus Macaques of Chinese Origin - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Background

Chronic immune activation is a hallmark of progressive HIV infection and a key determinant of immunodeficiency in HIV-infected individuals. Bacterial lipopolysaccharide LPS in the circulation has been implicated as a key factor in HIV infection-related systemic immune activation. We thus investigate the impact of LPS on systemic immune activation in simian immunodeficiency virus SIV-infected rhesus macaques of Chinese origin.

Methods

The animals were inoculated intravenously with SIVmac239. The levels of plasma viral load and host inflammatory cytokines in PBMC were measured by real-time RT-PCR. CD4-CD8 ratio and systemic immune activation markers were examined by flow cytometric analysis of PBMCs. White blood cell and neutrophil counts and C Reactive Protein levels were determined using biochemistry analyzer. The plasma levels of LPS were determined by Tachypleus Amebocyte Lysate TAL test.

Results

The animals inoculated with SIVmac239 became infected as evidenced by the increased plasma levels of SIV RNA and decreased CD4-CD8 ratio. LPS administration of SIV-infected animals induced a transient increase of plasma SIV RNA and immune activation, which was indicated by the elevated expression of the inflammatory cytokines and CD4+HLA-DR+ T cells in PBMCs.

Conclusions

These data support the concept that LPS is a driving factor in systemic immune activation of HIV disease.



Autor: Rong Bao, Ke Zhuang, Jinbiao Liu, Jianguo Wu, Jieliang Li, Xu Wang, Wen-Zhe Ho

Fuente: http://plos.srce.hr/



DESCARGAR PDF




Documentos relacionados