The Lantibiotic Lacticin 3147 Prevents Systemic Spread of Staphylococcus aureus in a Murine Infection Model - Download this document for free, or read online. Document in PDF available to download.

International Journal of MicrobiologyVolume 2012 2012, Article ID 806230, 6 pages

Research Article

Department of Microbiology, University College Cork, College Road, Cork, Ireland

Alimentary Pharmabiotic Centre, Cork, Ireland

Food Biosciences Department, Teagasc Food Research Centre, Moorepark, Fermoy, Cork, Ireland

Received 12 August 2011; Accepted 3 October 2011

Academic Editor: John Tagg

Copyright © 2012 Clare Piper et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The objective of this study was to investigate the in vivo activity of the lantibiotic lacticin 3147 against the luminescent Staphylococcus aureus strain Xen 29 using a murine model. Female BALB-c mice 7 weeks old, 17 g were divided into groups and infected with the Xen 29 strain via the intraperitoneal route at a dose of  cfu-animal. After 1.5 hr, the animals were treated subcutaneously with doses of phosphate-buffered saline PBS; negative control or lacticin 3147. Luminescent imaging was carried 3 and 5 hours postinfection. Mice were then sacrificed, and the levels of S. aureus Xen 29 in the liver, spleen, and kidneys were quantified. Notably, photoluminescence and culture-based analysis both revealed that lacticin 3147 successfully controlled the systemic spread of S. aureus in mice thus indicating that lacticin 3147 has potential as a chemotherapeutic agent for in vivo applications.





Author: Clare Piper, Pat G. Casey, Colin Hill, Paul D. Cotter, and R. Paul Ross

Source: https://www.hindawi.com/

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