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BioMed Research International - Volume 2016 2016, Article ID 1917394, 13 pages -

Research Article

Center for Infectious Disease and Cancer Research, Kaohsiung Medical University, Kaohsiung 807, Taiwan

Department of Pathology, Changhua Christian Hospital, Changhua 500, Taiwan

Department of Medical Imaging and Radiological Sciences, Kaohsiung Medical University, Kaohsiung 807, Taiwan

Department of Biomedical Engineering, National Yang-Ming University, Taipei 112, Taiwan

Department of Laboratory Medicine, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan

Department of Medical Laboratory Science and Biotechnology, College of Health Sciences, Kaohsiung Medical University, Kaohsiung 807, Taiwan

Office of Safety, Health and Environment, Kaohsiung Medical University, Kaohsiung 807, Taiwan

Mass Spectrometry Laboratory, Chemical, Molecular and Materials Analysis Center, Department of Chemistry, National University of Singapore, Singapore 119077

Department of Biomedical Engineering, Chung Yuan Christian University, Chungli 300, Taiwan

Department of Nuclear Medicine, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan

Department of Chemistry, National Sun Yat-sen University, Kaohsiung 804, Taiwan

Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan

Institute of Medical Science and Technology, National Sun Yat-sen University, Kaohsiung 804, Taiwan

Received 27 June 2016; Revised 10 September 2016; Accepted 5 October 2016

Academic Editor: Pornanong Aramwit

Copyright © 2016 Ming-Hui Yang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The microenvironment of neuron cells plays a crucial role in regulating neural development and regeneration. Hyaluronic acid HA biomaterial has been applied in a wide range of medical and biological fields and plays important roles in neural regeneration. PC12 cells have been reported to be capable of endogenous NGF synthesis and secretion. The purpose of this research was to assess the effect of HA biomaterial combining with PC12 cells conditioned media PC12 CM in neural regeneration. Using SH-SY5Y cells as an experimental model, we found that supporting with PC12 CM enhanced HA function in SH-SY5Y cell proliferation and adhesion. Through RP-nano-UPLC-ESI-MS-MS analyses, we identified increased expression of HSP60 and RanBP2 in SH-SY5Y cells grown on HA-modified surface with cotreatment of PC12 CM. Moreover, we also identified factors that were secreted from PC12 cells and may promote SH-SY5Y cell proliferation and adhesion. Here, we proposed a biomaterial surface enriched with neurotrophic factors for nerve regeneration application.





Autor: Ming-Hui Yang, Ko-Chin Chen, Pei-Wen Chiang, Tze-Wen Chung, Wan-Jou Chen, Pei-Yu Chu, Sharon Chia-Ju Chen, Yi-Shan Lu, Che

Fuente: https://www.hindawi.com/



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