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International Journal of Spectroscopy - Volume 2012 2012, Article ID 124672, 14 pages -

Review ArticleLaboratory of Microspectral Analysis of Cells and Cellular Systems and Optical Department Russian Academy of Sciences, Institute of Cell Biophysics, RAS, Institutskaya Str. 3, Pushchino, Moscow Region 142290, Russia

Received 22 March 2012; Revised 18 June 2012; Accepted 16 July 2012

Academic Editor: Jin Zhang

Copyright © 2012 Victoria V. Roshchina. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The application of various microscopy methods such as luminescence microscopy, microspectrofluorimetry and laser-scanning confocal microscopy has been considered as anapproach to study the autofluorescence of plant living cells—from cell diagnostics up to modelling the cell-cell contacts and cell interactions with fluorescent biologically active substances. It bases on the direct observations of secretions released from allelopathic and medicinal species and the cell-donor interactions with cell-acceptors as biosensors unicellular plant generative and vegetative microspores. Special attention was paid to the interactions with pigmented and fluorescing components of the secretions released by the cells-donors from plant species. Colored components of secretions are considered as histochemical dyes for the analysis of cellular mechanisms at the cell-cell contacts and modelling of cell-cell interactions. The fluorescence of plant biosensors was also recommended for the testing of natural plant excretions as medical drugs.

Author: Victoria V. Roshchina



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