Overexpression of SATB1 Is Associated with Biologic Behavior in Human Renal Cell CarcinomaReportar como inadecuado

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Special AT-rich sequence-binding protein-1 SATB1 has been reported to be aberrantly expressed in various cancers and correlated with the malignant behavior of cancer cells. However, the function of SATB1 in RCC remains unclear. With the combination of immunohistochemistry, western blotting, immunofluorescence, qRT-PCR, and cell proliferation, migration and invasion assays, we found that levels of SATB1 mRNA and protein were dramatically increased in human ccRCC tissues P<0.001 for both, and upregulation of SATB1 was significantly associated with depth of invasion P<0.001, lymph node status P = 0.001 and TNM stage P = 0.009. SATB1 knockdown inhibited the proliferation, migration and invasion of 786-O cells, whereas SATB1 overexpression promoted the growth and aggressive phenotype of ACHN cells in vitro. Furthermore, SATB1 expression was positively correlated with ZEB2 expression P = 0.013, and inversely linked to levels of SATB2 and E-cadherin P = 0.005 and P<0.001, respectively in ccRCC tissues. Our data provide a basis for the concept that overexpression of SATB1 may play a critical role in the acquisition of an aggressive phenotype for RCC cells through EMT, providing new insights into the significance of SATB1 in invasion and metastasis of ccRCC, which may contribute to fully elucidating the exact mechanism of development and progression of RCC.

Autor: Chao Cheng , Feng Wan , Lian Liu, Fuqing Zeng, Shi-an Xing, Xiaofei Wu, Xuepan Chen, Zhaohui Zhu

Fuente: http://plos.srce.hr/


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