Protective Effect of Indole-3-Pyruvate against Ultraviolet B-Induced Damage to Cultured HaCaT Keratinocytes and the Skin of Hairless MiceReportar como inadecuado




Protective Effect of Indole-3-Pyruvate against Ultraviolet B-Induced Damage to Cultured HaCaT Keratinocytes and the Skin of Hairless Mice - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Previous investigations demonstrated that pyruvate protects human keratinocytes against cell damage stemming from exposure to ultraviolet B UVB radiation. This study endeavoured to elucidate the protective capacity of aromatic pyruvates e.g., phenylpyruvate PPyr, 4-hydroxyphenylpyruvate HPPyr, and indole-3-pyruvate IPyr against UVB-induced injury to skin cells, both in vitro and in vivo. Cultured human HaCaT keratinocytes were irradiated with UVB light 60 mJ-cm2 and maintained with or without test compounds 1–25 mM. In addition, the dorsal skin of hairless mice HR-1 was treated with test compounds 100 µmol and exposed to UVB light 1 J-cm2 for two times. The ability of the test compounds to ameliorate UVB-induced cytotoxicity and inflammation was then assessed. Aromatic pyruvates reduced cytotoxicity in UVB-irradiated HaCaT keratinocytes, and also diminished the expression of interleukin 1β IL-1β and interleukin 6 IL-6. IPyr was more efficacious than either PPyr or HPPyr. Furthermore, only IPyr inhibited cyclooxygenase-2 Cox-2 expression at both the mRNA and the protein level in UVB-treated keratinocytes. Topical application of IPyr to the dorsal skin of hairless mice reduced the severity of UVB-induced skin lesions, the augmentation of dermal thickness, and transepithelial water loss. Overproduction of IL-1β and IL-6 in response to UVB radiation was also suppressed in vivo by the topical administration of IPyr. These data strongly suggest that IPyr might find utility as a UVB-blocking reagent in therapeutic strategies to lessen UVB-induced inflammatory skin damage.



Autor: Reiji Aoki, Ayako Aoki-Yoshida, Chise Suzuki, Yoshiharu Takayama

Fuente: http://plos.srce.hr/



DESCARGAR PDF




Documentos relacionados