Deeper Penetration of Erythrocytes into the Endothelial Glycocalyx Is Associated with Impaired Microvascular PerfusionReportar como inadecuado




Deeper Penetration of Erythrocytes into the Endothelial Glycocalyx Is Associated with Impaired Microvascular Perfusion - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Changes in endothelial glycocalyx are one of the earliest changes in development of cardiovascular disease. The endothelial glycocalyx is both an important biological modifier of interactions between flowing blood and the vessel wall, and a determinant of organ perfusion. We hypothesize that deeper penetration of erythrocytes into the glycocalyx is associated with reduced microvascular perfusion. The population-based prospective cohort study the Netherlands Epidemiology of Obesity NEO study includes 6,673 middle-aged individuals oversampling of overweight and obese individuals. Within this cohort, we have imaged the sublingual microvasculature of 915 participants using sidestream darkfield SDF imaging together with a recently developed automated acquisition and analysis approach. Presence of RBC as a marker of microvascular perfusion and perfused boundary region PBR, a marker for endothelial glycocalyx barrier properties for RBC accessibility, were assessed in vessels between 5 and 25 µm RBC column width. A wide range of variability in PBR measurements, with a mean PBR of 2.14 µm range: 1.43–2.86 µm, was observed. Linear regression analysis showed a marked association between PBR and microvascular perfusion, reflected by RBC filling percentage regression coefficient β: −0.034; 95% confidence interval: −0.037 to −0.031. We conclude that microvascular beds with a thick -healthy- glycocalyx low PBR, reflects efficient perfusion of the microvascular bed. In contrast, a thin -risk- glycocalyx high PBR is associated with a less efficient and defective microvascular perfusion.



Autor: Dae Hyun Lee, Martijn J. C. Dane, Bernard M. van den Berg, Margien G. S. Boels, Jurgen W. van Teeffelen, Renée de Mutsert, Marti

Fuente: http://plos.srce.hr/



DESCARGAR PDF




Documentos relacionados