A Combination of Serological Assays to Detect Human Antibodies to the Avian Influenza A H7N9 VirusReportar como inadecuado




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Human infection with avian influenza A H7N9 virus was first identified in March 2013 and represents an ongoing threat to public health. There is a need to optimize serological methods for this new influenza virus. Here, we compared the sensitivity and specificity of the hemagglutinin inhibition HI, microneutralization MN, and Western blot WB assays for the detection of human antibodies against avian influenza A H7N9 virus. HI with horse erythrocytes hRBCs and a modified MN assay possessed greater sensitivity than turkey erythrocytes and the standard MN assay, respectively. Using these assays, 80% of tested sera from confirmed H7N9 cases developed detectable antibody to H7N9 after 21 days. To balance sensitivity and specificity, we found serum titers of ≥20 MN or 160 HI samples were most effective in determining seropositive to H7N9 virus. Single serum with HI titers of 20–80 or MN titer of 10 could be validated by each other or WB assay. Unlike serum collected from adult or elderly populations, the antibody response in children with mild disease was low or undetectable. These combinations of assays will be useful in case diagnosis and serologic investigation of human cases.



Autor: Libo Dong , Hong Bo , Tian Bai , Rongbao Gao, Jie Dong, Ye Zhang, Junfeng Guo, Shumei Zou, Jianfang Zhou, Yun Zhu, Li Xin, Xiaoda

Fuente: http://plos.srce.hr/



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