Genome-Wide Analysis of Copy Number Variation Identifies Candidate Gene Loci Associated with the Progression of Non-Alcoholic Fatty Liver DiseaseReportar como inadecuado




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Between 10 and 25% of individuals with non-alcoholic fatty liver disease NAFLD develop hepatic fibrosis leading to cirrhosis and hepatocellular carcinoma HCC. To investigate the molecular basis of disease progression, we performed a genome-wide analysis of copy number variation CNV in a total of 49 patients with NAFLD 10 simple steatosis and 39 non-alcoholic steatohepatitis NASH and 49 matched controls using high-density comparative genomic hybridization CGH microarrays. A total of 11 CNVs were found to be unique to individuals with simple steatosis, whilst 22 were common between simple steatosis and NASH, and 224 were unique to NASH. We postulated that these CNVs could be involved in the pathogenesis of NAFLD progression. After stringent filtering, we identified four rare and-or novel CNVs that may influence the pathogenesis of NASH. Two of these CNVs, located at 13q12.11 and 12q13.2 respectively, harbour the exportin 4 XPO4 and phosphodiesterase 1B PDE1B genes which are already known to be involved in the etiology of liver cirrhosis and HCC. Cross-comparison of the genes located at these four CNV loci with genes already known to be associated with NAFLD yielded a set of genes associated with shared biological processes including cell death, the key process involved in ‘second hit’ hepatic injury. To our knowledge, this pilot study is the first to provide CNV information of potential relevance to the NAFLD spectrum. These data could prove invaluable in predicting patients at risk of developing NAFLD and more importantly, those who will subsequently progress to NASH.



Autor: Shamsul Mohd Zain , Rosmawati Mohamed , David N. Cooper, Rozaimi Razali, Sanjay Rampal, Sanjiv Mahadeva, Wah-Kheong Chan, Arif An

Fuente: http://plos.srce.hr/



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