MAPK Pathways Are Involved in Neuropathic Pain in Rats with Chronic Compression of the Dorsal Root GanglionReportar como inadecuado




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Evidence-Based Complementary and Alternative Medicine - Volume 2016 2016, Article ID 6153215, 8 pages -

Research ArticleDepartment of Physical Medicine and Rehabilitation, Qilu Hospital, Shandong University, Jinan, Shandong 250012, China

Received 21 December 2015; Accepted 28 June 2016

Academic Editor: Haroon Khan

Copyright © 2016 Yu-Juan Qu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The aim of the present study was to investigate whether the MAPK pathways were involved in the mechanism of neuropathic pain in rats with chronic compression of the dorsal root ganglion. We determined the paw withdrawal mechanical threshold PWMT of rats before and after CCD surgery and then after p38, JNK, or ERK inhibitors administration. Western blotting, RT-PCR, and immunofluorescence of dorsal root ganglia were performed to investigate the protein and mRNA level of MAPKs and also the alternation in distributions of positive neurons in dorsal root ganglia. Intrathecal administration of MAPKs inhibitors, SB203580 p38 inhibitor, SP600125 JNK inhibitor, and U0126 ERK inhibitor, resulted in a partial reduction in CCD-induced mechanical allodynia. The reduction of allodynia was associated with significant depression in the level of both MAPKs mRNA and protein expression in CCD rats and also associated with the decreased ratios of large size MAPKs positive neurons in dorsal root ganglia. In conclusion, the specific inhibitors of MAPKs contributed to the attenuation of mechanical allodynia in CCD rats and the large size MAPKs positive neurons in dorsal root ganglia were crucial.





Autor: Yu-Juan Qu, Lei Jia, Xiao Zhang, Hui Wei, and Shou-Wei Yue

Fuente: https://www.hindawi.com/



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