Formula Compatibility Identification of Dachengqi Decoction Based on the Effects of Absorbed Components in Cerulein-Injured Pancreatic AR42J CellsReportar como inadecuado




Formula Compatibility Identification of Dachengqi Decoction Based on the Effects of Absorbed Components in Cerulein-Injured Pancreatic AR42J Cells - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Evidence-Based Complementary and Alternative Medicine - Volume 2016 2016, Article ID 3198549, 8 pages -

Research Article

Sichuan Provincial Pancreatitis Center, Department of Integrative Medicine, West China Hospital, Sichuan University, Chengdu 610041, China

Department of Intensive Care Medicine, Sichuan Integrative Medicine Hospital, Chengdu 610041, China

Department of General Practice, Sichuan Provincial People’s Hospital, Chengdu 610072, China

Department of Pharmacology, School of Preclinical and Forensic Medicine, West China Medical Center, Sichuan University, Chengdu 610041, China

Received 13 November 2015; Revised 19 January 2016; Accepted 28 January 2016

Academic Editor: Hyunsu Bae

Copyright © 2016 Yumei Zhang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective. To identify the herbal formula compatibility law based on the effects of the absorbed components from DCQD on the cerulein-injured AR42J cells. Methods. AR42J cells were pretreated for 30 min with or without the different concentrations of the absorbed components from DCQD individually or in combination or DCQD and coincubated with cerulein 10 nM for a further 24 h. Cell viability, lactate dehydrogenase LDH release, and the levels of apoptosis and necrosis were measured. Results. Compared to DCQD, the individual or combination components partially protected cerulein-injured AR42J cells by increasing cell viability, reducing LDH release, and promoting apoptosis. Rhein, naringin, and honokiol were the main absorbed components from DCQD in cerulein-induced pancreatitis. Moreover, rhein in combination with naringin and honokiol had synergistic effects in protecting cerulein-injured AR42J cells and was better than the individual or the pairwise combination of the three components. Conclusions. The ten effective components from DCQD may elicit similar protective effects as DCQD on cerulein-induced pancreatitis. The principle of the formula compatibility of DCQD may be identified based on the effects of its absorbed components in cerulein-injured AR42J cells.





Autor: Yumei Zhang, Lin Zhu, Jia Wang, Jianlei Zhao, Xianlin Zhao, Hui Guo, Juan Li, and Wenfu Tang

Fuente: https://www.hindawi.com/



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