Survival, Growth and Reproduction of Cryopreserved Larvae from a Marine Invertebrate, the Pacific Oyster Crassostrea gigasReportar como inadecuado




Survival, Growth and Reproduction of Cryopreserved Larvae from a Marine Invertebrate, the Pacific Oyster Crassostrea gigas - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

This study is the first demonstration of successful post-thawing development to reproduction stage of diploid cryopreserved larvae in an aquatic invertebrate. Survival, growth and reproductive performances were studied in juvenile and adult Pacific oysters grown from cryopreserved embryos. Cryopreservation was performed at three early stages: trochophore 13±2 hours post fertilization: hpf, early D-larvae 24±2 hpf and late D-larvae 43±2 hpf. From the beginning 88 days at the end of the ongrowing phase 195 days, no mortality was recorded and mean body weights did not differ between the thawed oysters and the control. At the end of the growing-out phase 982 days, survival of the oysters cryopreserved at 13±2 hpf and at 43±2 hpf was significantly higher P<0.001 than those of the control non cryopreserved larvae. Only the batches cryopreserved at 24±2 hpf showed lower survival than the control. Reproductive integrity of the mature oysters, formely cryopreserved at 13±2 hpf and 24±2 hpf, was estimated by the sperm movement and the larval development of their offspring in 13 crosses gamete pools five males and five females in each pool. In all but two crosses out of 13 tested P<0.001, development rates of the offspring were not significantly different between frozen and unfrozen parents. In all, the growth and reproductive performances of oysters formerly cryopreserved at larval stages are close to those of controls. Furthermore, these performances did not differ between the three initial larval stages of cryopreservation. The utility of larvae cryopreservation is discussed and compared with the cryopreservation of gametes as a technique for selection programs and shellfish cryobanking.



Autor: Marc Suquet , Catherine Labbé, Sophie Puyo, Christian Mingant, Benjamin Quittet, Myrina Boulais, Isabelle Queau, Dominique Ratis

Fuente: http://plos.srce.hr/



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