Abnormal Behaviors and Developmental Disorder of Hippocampus in Zinc Finger Protein 521 ZFP521 Mutant MiceReportar como inadecuado




Abnormal Behaviors and Developmental Disorder of Hippocampus in Zinc Finger Protein 521 ZFP521 Mutant Mice - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Zinc finger protein 521 ZFP521 regulates a number of cellular processes in a wide range of tissues, such as osteoblast formation and adipose commitment and differentiation. In the field of neurobiology, it is reported to be an essential factor for transition of epiblast stem cells into neural progenitors in vitro. However, the role of ZFP521 in the brain in vivo still remains elusive. To elucidate the role of ZFP521 in the mouse brain, we generated mice lacking exon 4 of the ZFP521 gene. The birth ratio of our ZFP521Δ-Δ mice was consistent with Mendel-s laws. Although ZFP521Δ-Δ pups had no apparent defect in the body and were indistinguishable from ZFP521+-+ and ZFP521+-Δ littermates at the time of birth, ZFP521Δ-Δ mice displayed significant weight reduction as they grew, and most of them died before 10 weeks of age. They displayed abnormal behavior, such as hyper-locomotion, lower anxiety and impaired learning, which correspond to the symptoms of schizophrenia. The border of the granular cell layer of the dentate gyrus in the hippocampus of the mice was indistinct and granular neurons were reduced in number. Furthermore, Sox1-positive neural progenitor cells in the dentate gyrus and cerebellum were significantly reduced in number. Taken together, these findings indicate that ZFP521 directly or indirectly affects the formation of the neuronal cell layers of the dentate gyrus in the hippocampus, and thus ZFP521Δ-Δ mice displayed schizophrenia-relevant symptoms. ZFP521Δ-Δ mice may be a useful research tool as an animal model of schizophrenia.



Autor: Nobutaka Ohkubo , Etsuko Matsubara, Jun Yamanouchi, Rie Akazawa, Mamoru Aoto, Yoji Suzuki, Ikuya Sakai, Takaya Abe, Hiroshi Kiyon

Fuente: http://plos.srce.hr/



DESCARGAR PDF




Documentos relacionados