Bactericidal Antibiotics Increase Hydroxyphenyl Fluorescein Signal by Altering Cell MorphologyReportar como inadecuado




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It was recently proposed that for bactericidal antibiotics a common killing mechanism contributes to lethality involving indirect stimulation of hydroxyl radical OH• formation. Flow cytometric detection of OH• by hydroxyphenyl fluorescein HPF probe oxidation was used to support this hypothesis. Here we show that increased HPF signals in antibiotics-exposed bacterial cells are explained by fluorescence associated with increased cell size, and do not reflect reactive oxygen species ROS concentration. Independently of antibiotics, increased fluorescence was seen for elongated cells expressing the oxidative insensitive green fluorescent protein GFP. Although our data question the role of ROS in lethality of antibiotics other research approaches point to important interplays between basic bacterial metabolism and antibiotic susceptibility. To underpin such relationships, methods for detecting bacterial metabolites at a cellular level are needed.



Autor: Wilhelm Paulander , Ying Wang , Anders Folkesson, Godefroid Charbon, Anders Løbner-Olesen, Hanne Ingmer

Fuente: http://plos.srce.hr/



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