Generation of Leukemia Inhibitory Factor-Dependent Induced Pluripotent Stem Cells from the Massachusetts General Hospital Miniature PigReportar como inadecuado




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BioMed Research InternationalVolume 2013 2013, Article ID 140639, 11 pages

Research Article

Animal Biotechnology Division, National Institute of Animal Science, RDA, Suwon, Gyeonggi 441-706, Republic of Korea

Research Center of Integrative Cellulomics, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Republic of Korea

Department of Animal Biotechnology, Jeju National University, Jeju 690-756, Republic of Korea

Received 8 August 2013; Revised 21 October 2013; Accepted 21 October 2013

Academic Editor: Dong-Wook Han

Copyright © 2013 Dae-Jin Kwon et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The generation and application of porcine induced pluripotent stem cells iPSCs may enable the testing for safety and efficacy of therapy in the field of human regenerative medicine. Here, the generation of iPSCs from the Massachusetts General Hospital miniature pig MGH minipig established for organ transplantation studies is reported. Fibroblasts were isolated from the skin of the ear of a 10-day-old MGH minipig and transduced with a cocktail of six human factors: POU5F1, NANOG, SOX2, C-MYC, KLF4, and LIN28. Two distinct types of iPSCs were generated that were positive for alkaline phosphatase activity, as well as the classical pluripotency markers: Oct4, Nanog, Sox2, and the surface marker Ssea-1. Only one of two porcine iPSC lines differentiated into three germ layers both in vitro and in vivo. Western blot analysis showed that the porcine iPSCs were dependent on LIF or BMP-4 to sustain self-renewal and pluripotency. In conclusion, the results showed that human pluripotent factors could reprogram porcine ear fibroblasts into the pluripotent state. These cells may provide a useful source of cells that could be used for the treatment of degenerative and genetic diseases and agricultural research and application.





Autor: Dae-Jin Kwon, Hyelena Jeon, Keon Bong Oh, Sun-A Ock, Gi-Sun Im, Sung-Soo Lee, Seok Ki Im, Jeong-Woong Lee, Sung-Jong Oh, 

Fuente: https://www.hindawi.com/



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