Disrupted Endothelial Cell Layer and Exposed Extracellular Matrix Proteins Promote Capture of Late Outgrowth Endothelial Progenitor CellsReportar como inadecuado




Disrupted Endothelial Cell Layer and Exposed Extracellular Matrix Proteins Promote Capture of Late Outgrowth Endothelial Progenitor Cells - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Stem Cells International - Volume 2016 2016, Article ID 1406304, 13 pages -

Research ArticleDepartment of Medicine, Addenbrooke’s Hospital, University of Cambridge, Cambridge CB2 0QQ, UK

Received 7 March 2016; Revised 12 May 2016; Accepted 29 May 2016

Academic Editor: James Ankrum

Copyright © 2016 Jing Zhao et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Late outgrowth endothelial progenitor cells LO-EPC possess a high proliferative potential, differentiate into vascular endothelial cells EC, and form networks, suggesting they play a role in vascular repair. However, due to their scarcity in the circulation there is a requirement for ex vivo expansion before they could provide a practical cell therapy and it is currently unclear if they would home and engraft to an injury site. Using an in vitro flow system we studied LO-EPC under simulated injury conditions including EC activation, ischaemia, disrupted EC integrity, and exposed basement membrane. Perfused LO-EPC adhered to discontinuous EC paracellularly at junctional regions between adjacent cells under shear stress 0.7 dyn-cm

. The interaction was not adhesion molecule-dependent and not enhanced by EC activation. LO-EPC expressed high levels of the VE-Cadherin which may explain these findings. Ischaemia reperfusion injury decreased the interaction with LO-EPC due to cell retraction. LO-EPC interacted with exposed extracellular matrix ECM proteins, fibronectin and vitronectin. The interaction was mediated by integrins α5β3, αvβ1, and αvβ3. This study has demonstrated that an injured local environment presents sufficient adhesive signals to capture flow perfused LO-EPC in vitro and that LO-EPC have properties consistent with their potential role in vascular repair.





Autor: Jing Zhao, Claudia-Gabriela Mitrofan, Sarah L. Appleby, Nicholas W. Morrell, and Andrew M. L. Lever

Fuente: https://www.hindawi.com/



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