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Journal of Biomedicine and Biotechnology - Volume 2007 2007, Article ID 90651, 7 pages

Research Article

Division of Optical Sciences, U.S. Naval Research Laboratory, Washington, DC 20375, USA

Center for Bio-Molecular Science and Engineering, U.S. Naval Research Laboratory, Washington, DC 20375, USA

Promega Biosciences, Inc., 277 Granada Dr., San Luis Obispo, CA 93401, USA

Received 25 June 2007; Accepted 21 December 2007

Academic Editor: Marek Osinski

Copyright © 2007 Kimihiro Susumu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


We report the design and synthesis of a tetraethylene glycol- TEG- based bidentate ligand functionalized with dihydrolipoic acid DHLA and biotin DHLA—TEG—biotin to promote biocompatibility of luminescent quantum dots QD-s. This new ligand readily binds to CdSe—ZnS core-shell QDs via surface ligand exchange. QDs capped with a mixture of DHLA and DHLA—TEG—biotin or polyethylene glycol- PEG- molecular weight average ∼600 modified DHLA DHLA—PEG600 and DHLA—TEG—biotin are easily dispersed in aqueous buffer solutions. In particular, homogeneous buffer solutions of QDs capped with a mixture of DHLA—PEG600 and DHLA—TEG—biotin that are stable over broad pH range have been prepared. QDs coated with mixtures of DHLA-DHLA—TEG—biotin and with DHLA—PEG600-DHLA—TEG—biotin were tested in surface binding assays and the results indicate that biotin groups on the QD surface interact specifically with NeutrAvidin-functionalized microtiter well plates.

Autor: Kimihiro Susumu, H. Tetsuo Uyeda, Igor L. Medintz, and Hedi Mattoussi



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