Low Prevalence of Pneumococcal Carriage and High Serotype and Genotype Diversity among Adults over 60 Years of Age Living in PortugalReportar como inadecuado




Low Prevalence of Pneumococcal Carriage and High Serotype and Genotype Diversity among Adults over 60 Years of Age Living in Portugal - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Pneumococcal disease is frequent at the extremes of age. While several studies have looked at colonization among young children, much less is known among the elderly. We aimed to evaluate pneumococcal carriage among elderly adults living in Portugal. Between April 2010 and December 2012, nasopharyngeal and oropharyngeal swabs of adults over 60 years of age, living in an urban area n = 1,945 or in a rural area n = 1,416, were obtained. Pneumococci were isolated by culture-based standard procedures, identified by optochin susceptibility, bile solubility and PCR screening for lytA and cpsA, and characterized by antibiotype, serotype, and MLST. Associations between pneumococcal carriage, socio-demographic and clinical characteristics were evaluated by univariate analysis and multiple logistic regression. The global prevalence of carriage was 2.3% 95% CI: 1.8–2.8. In the multiple logistic regression analysis, smoking, being at a retirement home, and living in a rural area increased the odds of being a pneumococcal carrier by 4.4-fold 95% CI: 1.9–9.2, 2.0-fold 95% CI: 1.1–3.6 and 2.0-fold 95% CI: 1.2–3.5, respectively. Among the 77 pneumococcal isolates, 26 serotypes and 40 STs were identified. The most prevalent serotypes were in decreasing order 19A, 6C, 22F, 23A, 35F, 11A, and 23B, which accounted, in total, for 60.0% of the isolates. Most isolates 93.5% had STs previously described in the MLST database. Resistance to macrolides, non-susceptibility to penicillin and multidrug resistance were found in 19.5%, 11.7%, and 15.6% of the isolates, respectively. We conclude that the prevalence of pneumococcal carriage in the elderly, in Portugal, as determined by culture-based methods, is low. Serotype and genotype diversity is high. Living in a rural area, in a retirement home, and being a smoker increased the risk of pneumococcal carriage. This study contributes to the establishment of a baseline that may be used to monitor how novel pneumococcal vaccines impact on colonization among the elderly.



Autor: Sónia T. Almeida , Sónia Nunes , Ana Cristina Santos Paulo, Idalina Valadares, Sara Martins, Fátima Breia, António Brito-Avô

Fuente: http://plos.srce.hr/



DESCARGAR PDF




Documentos relacionados