Overexpression of OLC1 Promotes Tumorigenesis of Human Esophageal Squamous Cell CarcinomaReportar como inadecuado




Overexpression of OLC1 Promotes Tumorigenesis of Human Esophageal Squamous Cell Carcinoma - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Purpose

OLC1 was recently identified to be a potential oncogene. However, the role of OLC1 in human esophageal cell carcinoma ESCC is unknown. The aim of this study was therefore to evaluate the expression of OLC1 in human ESCC from normal, premalignant, and malignant lesions, and to clarify the mechanisms by which OLC1 contributes to the progression of ESCC.

Experimental Design

Two hundred and fourteen paired ESCC specimens, and an independent set from 28 ESCC patients, were used to analyze the correlation between OLC1 expression and the pathological characteristics of tumors using immunohistochemistry. Stable OLC1-overexpressing and OLC1-interfering esophageal cancer cells were established and a series of experimental methods were used to investigate the biological functions and mechanisms of action of OLC1.

Results

We showed that OLC1 was overexpressed in 145 of 214 67.8% of human ESCC specimens, compared with in only 59 of 214 27.57% paired adjacent normal tissues P<0.001. OLC1 overexpression occurred at a rate of 35% 10-28 at the stage of mild-moderate dysplasia, but was significantly upregulated to 66% 22-33 at the stages of severe dysplasia and in situ carcinoma, while 71% positive staining 22-28 was observed in invasive carcinoma tissues compared with normal tissues P<0.05. We also provided evidence that OLC1 abnormalities significantly altered the cell proliferation and apoptosis induced by cytotoxic agents. OLC1 overexpression suppressed apoptosis, and was associated with attenuated caspase-3 activation and increased Bcl-2 stability.

Conclusion

Our study provides strong evidence suggesting OLC1 abnormalities may contribute to the development of human ESCC and have some important clinical significance.



Autor: Xiao Li , Jing Suo , Shujuan Shao, Liyan Xue, Wei Chen, Lijia Dong, Ji Shi, Ming Fu, Ning Lu, Qimin Zhan , Tong Tong

Fuente: http://plos.srce.hr/



DESCARGAR PDF




Documentos relacionados