Circulating Angiogenic Cell Dysfunction in Patients with Hereditary Hemorrhagic TelangiectasiaReportar como inadecuado




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Hereditary hemorrhagic telangiectasia HHT is an autosomal dominant vascular disorder. Circulating angiogenic cells CACs play an important role in vascular repair and regeneration. This study was designed to examine the function of CACs derived from patients with HHT. Peripheral blood mononuclear cells PBMNCs isolated from patients with HHT and age- and gender-matched healthy volunteers were assessed for expression of CD34, CD133 and VEGF receptor 2 by flow cytometry. PBMNCs were cultured to procure early outgrowth CACs. Development of endothelial cell EC phenotype in CACs was analyzed by fluorescence microscopy. CAC apoptosis was assayed with Annexin V staining, and CAC migration assessed by a modified Boyden chamber assay. mRNA expression of endoglin ENG, activin receptor-like kinase-1 ACVLR1 or ALK1 and endothelial nitric oxide synthase eNOS in CACs was measured by real time RT-PCR. The percentage of CD34+ cells in PBMNCs from HHT patients was significantly higher than in PBMNCs of healthy controls. CACs derived from patients with HHT not only showed a significant reduction in EC-selective surface markers following 7-day culture, but also a significant increase in the rate of basal apoptosis and blunted migration in response to vascular endothelial growth factor and stromal cell-derived factor-1. CACs from HHT patients expressed significantly lower levels of ENG, ALK1 and eNOS mRNAs. In conclusion, CACs from patients with HHT exhibited various functional impairments, suggesting a reduced regenerative capacity of CACs to repair the vascular lesions seen in HHT patients.



Autor: Liana Zucco , Qiuwang Zhang , Michael A. Kuliszewski, Ivana Kandic, Marie E. Faughnan, Duncan J. Stewart, Michael J. Kutryk

Fuente: http://plos.srce.hr/



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