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Croatian journal of food science and technology, Vol.5 No.2 December 2013. -

The crude polysaccharide extracted from the flowers of burley tobacco hybrid Eyan 1 was fractionated on a Sepharose CL-6B chromatography to get two purified polysaccharide fractions termed Fr-I and Fr-II. The monocarbohydrate compositions revealed both fractions were heteropolysaccharide. Fr-I was mainly composed of mannose, galactose, ribose and arabinose, while Fr-II was composed of mannose, galactose and glucose. The IR spectrum of Fr-I and Fr-II revealed the typical characteristics with β-configuration. Furthermore, TGA Thermogravimetric analysis indicated the degradation temperature Td of the Fr-I and Fr-II was 210 °C and 185 °C, respectively. Both Fr-I and Fr-II possessed good scavenging abilities on hydroxyl radicals IC50 8.26 mg-ml and 6.23 mg-ml, respectively and DPPH radicals IC50 20.3±1.20 µg-ml, 17.5±1.28 µg-ml, respectively. Fr-II showed higher antioxidant properties than Fr-I p < 0.05. In a 13C NMR analysis of Fr-II, three anomeric peaks appeared at 102.95, 95.79 and 91.85 ppm, which were assigned to the mannose C-1, galactose C-1 and glucose C-1 residues, respectively.

extraction; purification; polysaccharide; antioxidant activity; tobacco flower

Autor: Chun-Ping Xu - ; College of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou, Henan, 450002, P.



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