Inhibitory Effects of Palm Tocotrienol-Rich Fraction Supplementation on Bilirubin-Metabolizing Enzymes in Hyperbilirubinemic Adult RatsReportar como inadecuado




Inhibitory Effects of Palm Tocotrienol-Rich Fraction Supplementation on Bilirubin-Metabolizing Enzymes in Hyperbilirubinemic Adult Rats - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Background

Phenylhydrazine, a hemolytic agent, is widely used as a model of experimental hyperbilirubinemia. Palm tocotrienol-rich fraction TRF was shown to exert beneficial effects in hyperbilirubinemic rat neonates.

Aim

To investigate the effects of palm TRF supplementation on hepatic bilirubin-metabolizing enzymes and ocidative stress status in rats administered phenylhydrazine.

Methods

Twenty-four male Wistar rats were divided into two groups; one group was intraperitoneally injected with palm TRF at the dose of 30 mg-kg-day, while another group was only given vehicle control vitamin E-free palm oil for 14 days. Twenty-four hours after the last dose, each group was further subdivided into another two groups. One group was administered phenylhydrazine 100 mg-kg, intraperitoneally and another group was administered normal saline. Twenty-four hours later, blood and liver were collected for biochemical parameter measurements.

Results

Phenylhydrazine increased plasma total bilirubin level and oxidative stress in the erythrocytes as well as in the liver, which were reduced by the pretreatment of palm TRF. Palm TRF also prevented the increases in hepatic heme oxygenase, biliverdin reductase and UDP-glucuronyltransferase activities induced by phenylhydrazine.

Conclusion

Palm tocotrienol-rich fraction was able to afford protection against phenylhydrazine-induced hyperbilirubinemia, possibly by reducing oxidative stress and inhibiting bilirubin-metabolizing enzymes in the liver.



Autor: Yusof Kamisah , Jing Jye Lim, Chew-Lian Lim, Ahmad Y. Asmadi

Fuente: http://plos.srce.hr/



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