Dually Fluorescent Core-Shell Microgels for Ratiometric Imaging in Live Antigen-Presenting CellsReportar como inadecuado




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Core-shell microgels containing sensors-dyes in a matrix were fabricated by two-stage free radical precipitation polymerization method for ratiometric sensing-imaging. The microgels composing of polyN-isopropylacrylamide PNIPAm shell exhibits a low critical solution temperature LCST, underwent an entropically driven transition from a swollen state to a deswollen state, which exhibit a hydrodynamic radius of ∼450 nm at 25°C in vitro and ∼190 nm at 37°C in vivo. The microgel’s ability of escaping from lysosome into cytosol makes the microgel be a potential candidate for cytosolic delivery of sensors-probes. Non-invasive imaging-sensing in Antigen-presenting cells APCs was feasible by monitoring the changes of fluorescence intensity ratios. Thus, these biocompatible microgels-based imaging-sensing agents may be expected to expand current molecular imaging-sensing techniques into methods applicable to studies in vivo, which could further drive APC-based treatments.



Autor: Xianfeng Zhou, Fengyu Su, Yanqing Tian , Deirdre R. Meldrum

Fuente: http://plos.srce.hr/



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