Decreased Expression of the GATA3 Gene Is Associated with Poor Prognosis in Primary Gastric AdenocarcinomaReportar como inadecuado




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Background

GATA binding protein 3 GATA3 was recently proposed to function as a tumor suppressor gene in some types of human cancer. This study aims to investigate GATA3 expression and its prognostic significance in primary gastric adenocarcinoma.

Methodology-Principal Findings

Using real-time quantitative PCR RT-qPCR and immunohistochemical staining methods, GATA3 expression was analyzed in tissue samples from a consecutive series of 402 gastric adenocarcinoma patients who underwent resections between 2003 and 2006. The relationship between GATA3 expression, clinicopathological factors, and patient survival was investigated. The expression status of GATA3 was shown to be clearly reduced in the tumor tissue samples compared with that in the matched adjacent non-tumor tissue samples by RT-qPCR P = 0.0014. Immunohistochemistry analysis indicated that GATA3 expression was significantly decreased in 225 of the 402 56% gastric adenocarcinoma cases. Reduced GATA3 expression was also observed in patients with large tumors P = 0.017, signet ring cell carcinoma or mucinous carcinoma P = 0.005 and tumors with lymphatic or venous invasion P = 0.040. Additionally, reduced expression of GATA3 was more commonly observed in tumors that were staged as T4a-b P<0.001, N3 P<0.001, or M1 P<0.001. Kaplan-Meier survival curves revealed that reduced expression of GATA3 was associated with poor prognosis in gastric adenocarcinoma patients P<0.001. Multivariate Cox analysis identified GATA3 expression as an independent prognostic factor for overall survival HR = 5.375, 95% CI = 3.647–7.921, P<0.001. To investigate the predictive ability of the models with and without containing GATA3 gene expression, Harrell-s c-index was calculated as a measure of predictive accuracy of survival outcome. The c-index values revealed that model containing GATA3 expression c-index = 0.897 had superior discrimination ability to the model without containg it c-index = 0.811.

Conclusions-Significance

Our data suggest that GATA3 plays an important role in tumor progression and that reduced GATA3 expression independently predicts an unfavorable prognosis in primary gastric adenocarcinoma patients.



Autor: Rajiv Prasad Keshari , Wei Wang , Yu Zhang, Dan-dan Wang, Yuan-fang Li, Shu-qiang Yuan, Hai-bo Qiu, Chun-yu Huang, Yong-ming Chen

Fuente: http://plos.srce.hr/



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