Microbial Diversity of Emalahleni Mine Water in South Africa and Tolerance Ability of the Predominant Organism to Vanadium and NickelReportar como inadecuado




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The present study aims firstly at determining the microbial diversity of mine-water collected in Emalahleni, South Africa and secondly isolating and characterizing the most dominant bacterial species found in the mine water in terms of its resistance to both V5+ and Ni2+ in a modified wastewater liquid media. The results revealed a microbial diversity of 17 orders, 27 families and 33 genera were found in the mine-water samples with Marinobacteria 47.02% and Anabaena 17.66% being the most abundant genera. Considering their abundance in the mine-water samples, a species of the Marinobacter genera was isolated, identified, and characterised for metal tolerance and removal ability. The MWI-1 isolate Marinobacter sp. MWI-1 AB793286 was found to be closely related to Marinobacter goseongensis at 97% of similarity. The isolate was exposed to various concentrations of Ni2+ and V5+ in wastewater liquid media and its tolerance to metals was also assessed. The MWI-1 isolate could tolerate V5+ and Ni2+ separately at concentrations in terms of MIC up to 13.41±0.56 mM and 5.39±0.5 mM at pH 7, whereas at pH 3, the tolerance limit decrease to 11.45±0.57 mM and 2.67±0.1 mM, respectively. The removal of V5+ and Ni2+ in liquid media was noted to gradually decrease with a gradual increase of the test metals. A significant difference p<0.05 between V5+ and Ni2+ removal was noted. Marinobacter sp. MWI-1 achieved the maximum permissible limit of 0.1 mg-V5+-L prescribed by UN-FAO at 100 mg-L, while at 200 mg-L only V5+ was removed at approximately 95% and Ni2+ at 47%. This study suggests that mine-water indigenous microorganisms are the best solution for the remediation of polluted mine water.



Autor: Ilunga Kamika, Maggie N. B. Momba

Fuente: http://plos.srce.hr/



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