L1cam Is Crucial for Cell Locomotion and Terminal Translocation of the Soma in Radial Migration during Murine CorticogenesisReportar como inadecuado




L1cam Is Crucial for Cell Locomotion and Terminal Translocation of the Soma in Radial Migration during Murine Corticogenesis - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

L1cam L1 is a cell adhesion molecule associated with a spectrum of human neurological diseases, the most well-known being X-linked hydrocephalus. Although we recently demonstrated that L1 plays an important role in neuronal migration during cortical histogenesis, the mechanisms of delayed migration have still not been clarified. In this study, we found that cell locomotion in the intermediate zone and terminal translocation in the primitive cortical zone PCZ were affected by L1-knockdown L1-KD. Time-lapse analyses revealed that L1-KD neurons produced by in utero electroporation of shRNA targeting L1 L1-shRNAs molecules showed decreased locomotion velocity in the intermediate zone, compared with control neurons. Furthermore, L1-KD neurons showed longer and more undulated leading processes during translocation through the primitive cortical zone. The curvature index, a quantitative index for curvilinearity, as well as the length of the leading process, were increased, whereas the somal movement was decreased in L1-KD neurons during terminal translocation in the PCZ. These results suggest that L1 has a role in radial migration of cortical neurons.



Autor: Madoka Tonosaki, Kyoko Itoh , Masafumi Umekage, Tomokazu Kishimoto, Takeshi Yaoi, Vance P. Lemmon, Shinji Fushiki

Fuente: http://plos.srce.hr/



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