Starvation Increases Insulin Sensitivity and Reduces Juvenile Hormone Synthesis in MosquitoesReportar como inadecuado




Starvation Increases Insulin Sensitivity and Reduces Juvenile Hormone Synthesis in Mosquitoes - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Background

The interactions between the insulin signaling pathway ISP and juvenile hormone JH controlling reproductive trade-offs are well documented in insects. JH and insulin regulate reproductive output in mosquitoes; both hormones are involved in a complex regulatory network, in which they influence each other and in which the mosquito-s nutritional status is a crucial determinant of the network-s output. Previous studies reported that the insulin-TOR target of rapamacyn signaling pathway is involved in the nutritional regulation of JH synthesis in female mosquitoes. The present studies further investigate the regulatory circuitry that controls both JH synthesis and reproductive output in response to nutrient availability.

Methods

We used a combination of diet restriction, RNA interference RNAi and insulin treatments to modify insulin signaling and study the cross-talk between insulin and JH in response to starvation. JH synthesis was analyzed using a newly developed assay utilizing fluorescent tags.

Conclusions

Our results reveal that starvation decreased JH synthesis via a decrease in insulin signaling in the corpora allata CA. Paradoxically, starvation-induced up regulation of insulin receptor transcripts and therefore -primed- the gland to respond rapidly to increases in insulin levels. During this response to starvation the synthetic potential of the CA remained unaffected, and the gland rapidly and efficiently responded to insulin stimulation by increasing JH synthesis to rates similar to those of CA from non-starved females.



Autor: Meritxell Perez-Hedo, Crisalejandra Rivera-Perez, Fernando G. Noriega

Fuente: http://plos.srce.hr/



DESCARGAR PDF




Documentos relacionados