Upregulation of Innate Antiviral Restricting Factor Expression in the Cord Blood and Decidual Tissue of HIV-Infected MothersReportar como inadecuado




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Programs for the prevention of mother-to-child transmission of HIV have reduced the transmission rate of perinatal HIV infection and have thereby increased the number of HIV-exposed uninfected HEU infants. Natural immunity to HIV-1 infection in both mothers and newborns needs to be further explored. In this study, we compared the expression of antiviral restricting factors in HIV-infected pregnant mothers treated with antiretroviral therapy ART in pregnancy n=23 and in cord blood CB n=16, placental tissues n=10-13 and colostrum n=5-6 samples and compared them to expression in samples from uninfected UN pregnant mothers n=21. Mononuclear cells MNCs were prepared from maternal and CB samples following deliveries by cesarean section. Maternal decidua and fetal chorionic villus placental tissues were obtained, and colostrum was collected 24 h after delivery. The mRNA and protein expression levels of antiviral factors were then evaluated. We observed a significant increase in the mRNA expression levels of antiviral factors in MNCs from HIV-infected mothers and CB, including the apolipoprotein B mRNA-editing enzyme 3G A3G, A3F, tripartite motif family-5α TRIM-5α, TRIM-22, myxovirus resistance protein A MxA, stimulator of interferon IFN genes STING and IFN-β, compared with the levels detected in uninfected UN mother-CB pairs. Moreover, A3G transcript and protein levels and α-defensin transcript levels were decreased in the decidua of HIV-infected mothers. Decreased TRIM-5α protein levels in the villi and increased STING mRNA expression in both placental tissues were also observed in HIV-infected mothers compared with uninfected UN mothers. Additionally, colostrum cells from infected mothers showed increased tetherin and IFN-β mRNA levels and CXCL9 protein levels. The data presented here indicate that antiviral restricting factor expression can be induced in utero in HIV-infected mothers. Future studies are warranted to determine whether this upregulation of antiviral factors during the perinatal period has a protective effect against HIV-1 infection.



Autor: Nátalli Zanete Pereira, Elaine Cristina Cardoso, Luanda Mara da Silva Oliveira, Josenilson Feitosa de Lima, Anna Cláudia Calvie

Fuente: http://plos.srce.hr/



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