Identification and Expression Analysis of Diapause Hormone and Pheromone Biosynthesis Activating Neuropeptide DH-PBAN in the Legume Pod Borer, Maruca vitrata FabriciusReportar como inadecuado




Identification and Expression Analysis of Diapause Hormone and Pheromone Biosynthesis Activating Neuropeptide DH-PBAN in the Legume Pod Borer, Maruca vitrata Fabricius - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Neuropeptides play essential roles in a variety of physiological responses that contribute to the development and reproduction of insects. Both the diapause hormone DH and pheromone biosynthesis activating neuropeptide PBAN belong to the PBAN-pyrokinin neuropeptide family, which has a conserved pentapeptide motif FXPRL at the C-terminus. We identified the full-length cDNA encoding DH-PBAN in Maruca vitrata, a major lepidopteran pest of leguminous crops. The open reading frame of Marvi-DH-PBAN is 591 bp in length, encoding 197 amino acids, from which five putative neuropeptides DH, PBAN, α-subesophageal ganglion neuropeptide SGNP, β-SGNP and γ-SGNP are derived. Marvi-DH-PBAN was highly similar 83% to DH-PBAN of Omphisa fuscidentalis Lepidoptera: Crambidae, but possesses a unique C-terminal FNPRL motif, where asparagine has replaced a serine residue present in other lepidopteran PBAN peptides. The genomic DNA sequence of Marvi-DH-PBAN is 6,231 bp in size and is composed of six exons. Phylogenetic analysis has revealed that the Marvi-DH-PBAN protein sequence is closest to its homolog in Crambidae, but distant from Diptera, Coleoptera and Hymenoptera DH-PBAN, which agrees with the current taxonomy. DH-PBAN transcripts were present in the head and thoracic complex, but absent in the abdomen of M. vitrata. Real-time quantitative PCR assays have demonstrated a relatively higher expression of Marvi-DH-PBAN mRNA in the latter half of the pupal stages and in adults. These findings represent a significant step forward in our understanding of the DH-PBAN gene architecture and phylogeny, and raise the possibility of using Marvi-DH-PBAN to manage M. vitrata populations through molecular techniques.



Autor: Jian-Cheng Chang, Srinivasan Ramasamy

Fuente: http://plos.srce.hr/



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