Role of IL-6 in Exercise Training- and Cold-Induced UCP1 Expression in Subcutaneous White Adipose TissueReportar como inadecuado

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Expression of brown adipose tissue BAT associated proteins like uncoupling protein 1 UCP1 in inguinal WAT iWAT has been suggested to alter iWAT metabolism. The aim of this study was to investigate the role of interleukin-6 IL-6 in exercise training and cold exposure-induced iWAT UCP1 expression. The effect of daily intraperitoneal injections of IL-6 3 ng-g in C57BL-6 mice for 7 days on iWAT UCP1 expression was examined. In addition, the expression of UCP1 in iWAT was determined in response to 3 days of cold exposure 4°C and 5 weeks of exercise training in wild type WT and whole body IL-6 knockout KO mice. Repeated injections of IL-6 in C57BL-6 mice increased UCP1 mRNA but not UCP1 protein content in iWAT. Cold exposure increased iWAT UCP1 mRNA content similarly in IL-6 KO and WT mice, while exercise training increased iWAT UCP1 mRNA in WT mice but not in IL-6 KO mice. Additionally, a cold exposure-induced increase in iWAT UCP1 protein content was blunted in IL-6 KO mice, while UCP1 protein content in iWAT was lower in both untrained and exercise trained IL-6 KO mice than in WT mice. In conclusion, repeated daily increases in plasma IL-6 can increase iWAT UCP1 mRNA content and IL-6 is required for an exercise training-induced increase in iWAT UCP1 mRNA content. In addition IL-6 is required for a full induction of UCP1 protein expression in response to cold exposure and influences the UCP1 protein content iWAT of both untrained and exercise trained animals.

Autor: Jakob G. Knudsen, Maria Murholm, Andrew L. Carey, Rasmus S. Biensø, Astrid L. Basse, Tamara L. Allen, Juan Hidalgo, Bronwyn A. K



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