A Non-Biological Method for Screening Active Components against Influenza Virus from Traditional Chinese Medicine by Coupling a LC Column with Oseltamivir Molecularly Imprinted PolymersReport as inadecuate




A Non-Biological Method for Screening Active Components against Influenza Virus from Traditional Chinese Medicine by Coupling a LC Column with Oseltamivir Molecularly Imprinted Polymers - Download this document for free, or read online. Document in PDF available to download.

To develop a non-biological method for screening active components against influenza virus from traditional Chinese medicine TCM extraction, a liquid chromatography LC column prepared with oseltamivir molecularly imprinted polymer OSMIP was employed with LC-mass spectrometry LC-MS. From chloroform extracts of compound TCM liquid preparation, we observed an affinitive component m-z 249, which was identified to be matrine following analysis of phytochemical literatures, OSMIP-LC column on-line of control compounds and MS-MS off-line. The results showed that matrine had similar bioactivities with OS against avian influenza virus H9N2 in vitro for both alleviating cytopathic effect and hemagglutination inhibition and that the stereostructures of these two compounds are similar while their two-dimensional structures were different. In addition, our results suggested that the bioactivities of those affinitive compounds were correlated with their chromatographic behaviors, in which less difference of the chromatographic behaviors might have more similar bioactivities. This indicates that matrine is a potential candidate drug to prevent or cure influenza for human or animal. In conclusion, the present study showed that molecularly imprinted polymers can be used as a non-biological method for screening active components against influenza virus from TCM.



Author: Ya-Jun Yang, Jian-Yong Li , Xi-Wang Liu, Ji-Yu Zhang, Yu-Rong Liu, Bing Li

Source: http://plos.srce.hr/



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